Méthode et outil de simulation distribuée pour l'accommodation de la conduite de réseaux d'entreprises

Aujourd'hui, la plupart des entreprises s'organisent en réseaux de partenaires, de fournisseurs et de soustraitants pour réaliser de nouveaux projets. Cependant, les outils classiques de planification n'offrent pas toutes les fonctionnalités nécessaires pour garantir la conduite de telles organisations. Dans ce mémoire, une démarche et un outil sont proposés pour évaluer la faisabilité des plans multi-sites de production ou de transports avant leur exécution. La méthode présentée permet d'adapter la configuration de ces réseaux afin de maintenir de bonnes performances pour les projets évalués. Intégrant le protocole HLA, l'outil gère la synchronisation des simulateurs en garantissant la causalité des événements simulés. La bonne exécution des plans multi-sites associés aux configurations d'ateliers et de logistique est ainsi évaluée. Les performances de l'outil révélées par les résultats de simulation de différents scénarios montrent l'intérêt et l'effectivité de l'approche. ABSTRACT : Currently, most of the companies are organized in networks of partners, suppliers and subcontractors to carry out new projects. However, the conventional tools for planning do not offer all the functionalities making it possible to guarantee the control of such organizations. In this dissertation, the steps and a tool are provided to evaluate the feasibility of the production or transportation multi-site plans before their execution. The presented method makes it possible to adapt the configuration of these networks in order to maintain good performances for the evaluated projects. Integrating the HLA protocol, the tool manages the synchronization of the simulators by guaranteeing the causality of the simulated events. The good execution of the multi-site plans associated to the workshops and logistics configurations is assessed. The tool performances revealed by the simulation results of various scenarios show the interest and the efficiency of the proposed approach

Human–Machine Interface in Transport Systems: An Industrial Overview for More Extended Rail Applications

This paper provides an overview of Human Machine Interface (HMI) design and command systems in commercial or experimental operation across transport modes. It presents and comments on different HMIs from the perspective of vehicle automation equipment and simulators of different application domains. Considering the fields of cognition and automation, this investigation highlights human factors and the experiences of different industries according to industrial and literature reviews. Moreover, to better focus the objectives and extend the investigated industrial panorama, the analysis covers the most effective simulators in operation across various transport modes for the training of operators as well as research in the fields of safety and ergonomics. Special focus is given to new technologies that are potentially applicable in future train cabins, e.g., visual displays and haptic-shared controls. Finally, a synthesis of human factors and their limits regarding support for monitoring or driving assistance is propose

Combined collision-induced dissociation and photo-selected reaction monitoring mass spectrometry modes for simultaneous analysis of coagulation factors and estrogens

AbstractOral estrogens are directly associated with changes in plasma levels of coagulation proteins. Thus, the detection of any variation in protein concentrations due to estrogen contraceptives, by a simultaneous analysis of both coagulation proteins and estrogens, would be a very informative tool. In the present study, the merit of photo-selected reaction monitoring (SRM), a new analytical tool, was evaluated towards estrogens detection in plasma. Then, SRM and photo-SRM detection modes were combined for the simultaneous analysis of estrogen molecules together with heparin co-factor and factor XIIa, two proteins involved in the coagulation cascade. This study shows that photo-SRM could open new multiplexed analytical routes

Autophagy supports Candida glabrata survival during phagocytosis

The opportunistic human fungal pathogen Candida glabrata is confronted with phagocytic cells of the host defence system. Survival of internalized cells is thought to contribute to successful dissemination. We investigated the reaction of engulfed C. glabrata cells using fluorescent protein fusions of the transcription factors CgYap1 and CgMig1 and catalase CgCta1. The expression level and peroxisomal localization of catalase was used to monitor the metabolic and stress status of internalized C. glabrata cells. These reporters revealed that the phagocytosed C. glabrata cells were exposed to transient oxidative stress and starved for carbon source. Cells trapped within macrophages increased their peroxisome numbers indicating a metabolic switch. Prolonged phagocytosis caused a pexophagy-mediated decline in peroxisome numbers. Autophagy, and in particular pexophagy, contributed to survival of C. glabrata during engulfment. Mutants lacking CgATG11 or CgATG17, genes required for pexophagy and non-selective autophagy, respectively, displayed reduced survival rates. Furthermore, both CgAtg11 and CgAtg17 contribute to survival, since the double mutant was highly sensitive to engulfment. Inhibition of peroxisome formation by deletion of CgPEX3 partially restored viability of CgATG11 deletion mutants during engulfment. This suggests that peroxisome formation and maintenance might sequester resources required for optimal survival. Mobilization of intracellular resources via autophagy is an important virulence factor that supports the viability of C. glabrata in the phagosomal compartment of infected innate immune cells

Méthode et outil de simulation distribuée pour l’accommodation de la conduite de réseaux d'entreprise

Method and distributed simulation tool for the control accomodation of corporate networksAujourd'hui, la plupart des entreprises s'organisent en réseaux de partenaires, de fournisseurs et de sous traitants pour réaliser de nouveaux projets. Cependant, les outils classiques de planification n'offrent pas toutes les fonctionnalités nécessaires pour garantir la conduite de telles organisations. Dans ce mémoire, une démarche et un outil sont proposés pour évaluer la faisabilité des plans multi-sites de production ou de transports avant leur exécution. La méthode présentée permet d'adapter la configuration de ces réseaux afin de maintenir de bonnes performances pour les projets évalués. Intégrant le protocole HLA, l'outil gère la synchronisation des simulateurs en garantissant la causalité des événements simulés. La bonne exécution des plans multi-sites associés aux configurations d'ateliers et de logistique est ainsi évaluée. Les performances de l'outil révélées par les résultats de simulation de différents scénarios montrent l'intérêt et l'effectivité de l'approche

Observer-Based Tracking design using H∞ criteria: Application to eco-driving in a tramway system

International audienceThis paper investigates an H∞ Observer-Based Controller design for tracking a tramway system eco-driving trajectory. The model of the tramway system is given in state space form, and the poor manoeuvres of the of the driver when following a reference trajectory are modeled as disturbances with finite energy that affect the system dynamics. To minimize the impact of poor driver manoeuvres, an H∞ Observer-Based Tracking Controller (H∞-OBTC) was designed and its conditions of existence are given. In addition, to ensure the robust convergence of the estimation and the tracking errors simultaneously, a new sufficient condition was obtained based on the Bounded Real Lemma. Two algorithms are presented to solve the robust stability condition obtained. The first one is based on a two-step procedure. Then a linearization approach was used to present the robust stability condition of the errors as a convex optimization problem with a Linear Matrix Inequality (LMI) constraint.The gain matrices of the H∞-OBTC can be computed by solving the LMI given, subject to a minimization constraint

Méthode et outil de simulation distribuée pour l'accommodation de la conduite de réseaux d'entreprises

Aujourd'hui, la plupart des entreprises s'organisent en réseaux de partenaires, de fournisseurs et de sous-traitants pour réaliser de nouveaux projets. Cependant, les outils classiques de planification n'offrent pas toutes les fonctionnalités nécessaires pour garantir la conduite de telles organisations. Dans ce mémoire, une démarche et un outil sont proposés pour évaluer la faisabilité des plans multi-sites de production ou de transports avant leur exécution. La méthode présentée permet d'adapter la configuration de ces réseaux afin de maintenir de bonnes performances pour les projets évalués. Intégrant le protocole HLA, l'outil gère la synchronisation des simulateurs en garantissant la causalité des évènements simulés. La bonne exécution des plans multi-sites associés aux configurations d'ateliers et de logistiques est ainsi évaluée. Les performances de l'outil révélées par les résultats de simulation de différents scénarios montrent l'intérêt et l'effectivité de l'approche.Currently, most of the companies are organized in networks of partners, suppliers and subcontractors to carry out new projects. However, the conventional tools for planning do not offer all the functionalities making it possible to guarantee the control of such organizations. In this dissertation, the steps and a tool are provided to evaluate the feasibility of the production or transportation multi-site plans before their execution. The presented method makes it possible to adapt the configuration of the networks in order to maintain good performances for the evaluated projects. Integrating the HLA protocol, the tool manages the synchronization of the simulators by guaranteeing the causality of the simulated events. The good execution of the multi-site plans associated to the workshops and logistics configurations is assessed. The tool performances revealed by the simulation results of various scenarios show the interest and the efficiency of the proposed approach.TOULOUSE-ENSEEIHT (315552331) / SudocTARBES-ENIT (654402301) / SudocSudocFranceF

Unified Driver Model simulation and its application to the automotive, rail and maritime domains

This paper describes the implementation of a model of a driver into a computerised numerical simulation. The model is developed to capture the essential characteristics and common aspects of cognition and behaviour of a human being in control of a "vehicle" in different surface transport systems, namely trains, cars and ships. The main functions of the simulation are discussed as well as the experiments carried out in different types of driving simulators to support the estimation of the parameters utilised in the numerical simulation. The validation processes carried out in the rail and maritime domains are also discussed together with a critical review of capacities and limitations of the proposed approach

Evaluation of hydrophilic interaction chromatography (HILIC) versus C-18 reversed-phase chromatography for targeted quantification of peptides by mass spectrometry

International audienceHydrophilic-interaction liquid chromatography (HILIC) is a widely used technique for small polar molecule analysis and offers the advantage of improved sensitivity in mass spectrometry. Although HILIC is today frequently employed as an orthogonal fractionation method for peptides during the proteomic discovery phase, it is still seldom considered for quantification. In this study, the performances in terms of peak capacity and sensitivity of 3 HILIC columns were compared to traditional reversed phase liquid C-18 column in the context of targeted quantification of proteotypic peptides using selected reaction monitoring mode (SRM). The results showed that the maximum sensitivity in HILIC chromatography was achieved by using an amide column without salt buffer and that the signal increased compared to classic reversed phase chromatography. However, the intensity improvement is quite low compared to the one obtained for small molecules. This is due on one hand to a higher matrix effect in HILIC and on the other hand to a change of charge states of peptides in organic solvent (doubly charged to monocharged). The doubly charged ions can be more readily dissociated than singly charged ions, making them ideal for SRM peptide quantification. As a result "supercharging" reagents are added to the mobile phase to shift from predominant singly charged ions to the more favorable doubly charged species. Using such optimized conditions, peptide signal is improved by a factor of between two and ten for 88% of the peptides of the 81 peptides investigate